Polydiacetylene-coated sensor strip for immunochromatic detection of Xylella fastidiosa subsp. fastidiosa


1Department of Mechanical Engineering, University of California, Riverside, CA, USA
2Department of Bioengineering, University of California, Riverside, CA, USA
3Department of Plant Pathology and Microbiology, University of California, Riverside, CA, USA


This study presents a sensor strip for user-friendly, naked-eye detection of Xylella fastidiosa, the bacterial causal agent of Pierce’s disease in grapevine. This sensor uses anti–X. fastidiosa antibodies conjugated to a polydiacetylene layer on a polyvinylidene fluoride strip to generate specific color transitions and discriminate levels of the pathogen. The detection limit of the sensor is 0.8 × 108 cells/mL, which is similar to bacterial load in grapevine 18 days following bacterial inoculation. This sensor enables equipment-free detection that is highly desirable for in-field diagnostic tools in resource-limited settings.

Published on January 31, 2017 by SLAS TECHNOLOGY