Draft Genome Sequence Resources of Three Strains (TOS4, TOS5, and TOS14) of Xylella fastidiosa Infecting Different Host Plants in the Newly Discovered Outbreak in Tuscany, Italy


Annalisa Giampetruzzi 1 , Giusy D’Attoma 2 , Stefania Zicca 2 , Raied Abou Kubaa 2 , Domenico Rizzo 3 , Donato Boscia 2 , Pasquale Saldarelli 2 , and Maria Saponari 2


1 Dipartimento di Scienze del Suolo della Pianta e degli Alimenti, Università degli Studi di Bari Aldo Moro, 70126 Bari, Italy
2 Istituto per la Protezione Sostenibile delle Piante, CNR, 70126 Bari, Italy
3 Regione Toscana, Servizio Fitosanitario Regionale e di Vigilanza e Controllo Agroforestale, 50121 Firenze, Italy


An outbreak of Xylella fastidiosa was discovered in late 2018 in northern Italy affecting several plant species. Multilocus sequence typing analyses detected the presence of strains clustering in X. fastidiosa subsp. multiplex and harboring a hitherto uncharacterized sequence type, ST87. Three cultured strains (TOS4, TOS5, and TOS14) were subjected to high-throughput sequencing and the draft genomes assembled. Phylogenetic analysis conclusively indicated that they belong to the subspecies multiplex. The genetic information generated for these newly discovered strains further supports the evidence that sequence types are associated with the emergence of X. fastidiosa in Europe, posing major challenges for predicting the main threatened European and Mediterranean crops and plant species.

This work was funded by Project XF-ACTORS (Xylella fastidiosa active containment through a multidisciplinary-oriented research strategy; grant ID number 727987) from the European Union’s Horizon 2020 Food research and innovation programme.

Published on July 19, 2019 by PHYTOPATHOLOGY